Extraction of high molecular weight DNA from microbial mats
نویسندگان
چکیده
منابع مشابه
Extraction of high molecular weight DNA from microbial mats.
Due to the presence of inhibitors such as extracellular polymeric substances (EPSs) and salts, most microbial mat studies have relied on harsh methods of direct DNA extraction that result in DNA fragments too small for large-insert vector cloning. High molecular weight (HMW) DNA is crucial in functional metagenomic studies, because large fragments present greater access to genes of interest. He...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2010
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113486